常见的荧光染料以及它们的激发发射波长大汇总！
作者：llh•来源：齐岳生物•浏览次数：651 •发布时间：2022-06-20 09:58:44
本文摘要：ProbeEx (nm)Em (nm)MWNotesReactive and conjugated probesHydroxycoumarin325386331Succinimidyl esterAm···
| Probe | Ex (nm) | Em (nm) | MW | Notes |
| Reactive and conjugated probes | ||||
| Hydroxycoumarin | 325 | 386 | 331 | Succinimidyl ester |
| Aminocoumarin | 350 | 445 | 330 | Succinimidyl ester |
| Methoxycoumarin | 360 | 410 | 317 | Succinimidyl ester |
| Cascade Blue | (375);401 | 423 | 596 | Hydrazide |
| Pacific Blue | 403 | 455 | 406 | Maleimide |
| Pacific Orange | 403 | 551 | ||
| Lucifer yellow | 425 | 528 | ||
| NBD | 466 | 539 | 294 | NBD-X |
| R-Phycoerythrin (PE) | 480;565 | 578 | 240 k | |
| PE-Cy5 conjugates | 480;565;650 | 670 | aka Cychrome, R670, Tri-Color, Quantum Red | |
| PE-Cy7 conjugates | 480;565;743 | 767 | ||
| Red 613 | 480;565 | 613 | PE-Texas Red | |
| PerCP | 490 | 675 | Peridinin chlorphyll protein | |
| TruRed | 490675 | 695 | PerCP-Cy5.5 conjugate | |
| FluorX | 494 | 520 | 587 | (GE Healthcare) |
| Fluorescein | 495 | 519 | 389 | FITC; pH sensitive |
| BODIPY-FL | 503 | 512 | ||
| TRITC | 547 | 572 | 444 | TRITC |
| X-Rhodamine | 570 | 576 | 548 | XRITC |
| Lissamine Rhodamine B | 570 | 590 | ||
| Texas Red | 589 | 615 | 625 | Sulfonyl chloride |
| Allophycocyanin (APC) | 650 | 660 | 104 k | |
| APC-Cy7 conjugates | 650;755 | 767 | PharRed | |
| Alexa Fluor dyes | ||||
| Alexa Fluor 350 | 343 | 442 | 410 | |
| Alexa Fluor 405 | 401 | 421 | 1028 | |
| Alexa Fluor 430 | 434 | 540 | 702 | |
| Alexa Fluor 488 | 499 | 519 | 643 | QY 0.92 |
| Alexa Fluor 500 | 503 | 525 | 700 | |
| Alexa Fluor 514 | 517 | 542 | 714 | |
| Alexa Fluor 532 | 530 | 555 | 724 | QY 0.61 |
| Alexa Fluor 546 | 561 | 572 | 1079 | QY 0.79 |
| Alexa Fluor 555 | 553 | 568 | 1250 | QY 0.1 |
| Alexa Fluor 568 | 579 | 603 | 792 | QY 0.69 |
| Alexa Fluor 594 | 591 | 618 | 820 | QY 0.66 |
| Alexa Fluor 610 | 610 | 629 | 1285 | |
| Alexa Fluor 633 | 632 | 648 | 1200 | |
| Alexa Fluor 647 | 652 | 668 | 1300 | QY 0.33 |
| Alexa Fluor 660 | 663 | 691 | 1100 | |
| Alexa Fluor 680 | 680 | 702 | 1150 | |
| Alexa Fluor 700 | 696 | 719 | 1400 | |
| Alexa Fluor 750 | 752 | 776 | 1300 | |
| Alexa Fluor 790 | 782 | 804 | 1750 | |
| Cy Dyes | ||||
| Cy2 | 489 | 506 | 714 | QY 0.12 |
| Cy3 | (512);550 | 570;(615) | 767 | QY 0.15 |
| Cy3B | 558 | 572;(620) | 658 | QY 0.67 |
| Cy3.5 | 581 | 594;(640) | 1102 | QY 0.15 |
| Cy5 | (625);650 | 670 | 792 | QY 0.28 |
| Cy5.5 | 675 | 694 | 1128 | QY 0.23 |
| Cy7 | 743 | 767 | 818 | QY 0.28 |
| DyLight dyes (Pierce) | ||||
| DyLight 350 | 353 | 432 | ||
| DyLight 405 | 400 | 420 | ||
| DyLight 488 | 493 | 518 | ||
| DyLight 549 | 562 | 576 | ||
| DyLight 594 | 593 | 618 | ||
| DyLight 633 | 638 | 658 | ||
| DyLight 649 | 654 | 673 | ||
| DyLight 680 | 692 | 712 | ||
| DyLight 750 | 752 | 778 | ||
| DyLight 800 | 777 | 794 | ||
| Nucleic acid probes | ||||
| Hoechst 33342 | 343 | 483 | 616 | AT-selective |
| DAPI | 345 | 455 | AT-selective | |
| Hoechst 33258 | 345 | 478 | 624 | AT-selective |
| SYTOX Blue | 431 | 480 | ~400 | DNA |
| Chromomycin A3 | 445 | 575 | CG-selective | |
| Mithramycin | 445 | 575 | ||
| YOYO-1 | 491 | 509 | 1271 | |
| Ethidium Bromide | 493 | 620 | 394 | |
| Acridine Orange | 503 | 530/640 | DNA/RNA | |
| SYTOX Green | 504 | 523 | ~600 | DNA |
| TOTO-1, TO-PRO-1 | 509 | 533 | Vital stain, TOTO: Cyanine Dimer | |
| TO-PRO: Cyanine Monomer | ||||
| Thiazole Orange | 510 | 530 | ||
| Propidium Iodide (PI) | 536 | 617 | 668.4 | |
| LDS 751 | 543;590 | 712;607 | 472 | DNA (543ex/712em), RNA (590ex/607em) |
| 7-AAD | 546 | 647 | 7-aminoactinomycin D, CG-selective | |
| SYTOX Orange | 547 | 570 | ~500 | DNA |
| TOTO-3, TO-PRO-3 | 642 | 661 | ||
| DRAQ5 | 647 | 681697 | 413 | (Biostatus) (usable excitation down to 488) |
| Cell function probes | ||||
| Indo-1 | 361/330 | 490/405 | 1010 | AM ester. Low/High Ca++, |
| Fluo-3 | 506 | 526 | 855 | AM ester. pH > 6 |
| DCFH | 505 | 535 | 529 | 2'7'Dichorodihydrofluorescein, oxidized form |
| DHR | 505 | 534 | 346 | Dihydrorhodamine 123, oxidized form,light catalyzes oxidation |
| SNARF | 548/579 | 587/635 | pH 6/9 | |
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